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Kinetics of determination in the differentiation of isolated mesophyll cells of Zinnia elegans to tracheary elementsMechanically isolated mesophyll cells of Zinnia elegans L. cv Envy differentiate to tracheary elements when cultured in inductive medium containing 0.5 micromolar alpha-naphthaleneacetic acid and 0.5 micromolar benzyladenine. The cells do not differentiate when cultured in medium in which the concentration of auxin and/or cytokinin has been reduced to 0.005 micromolar. Cells require an initial 24-hour exposure to inductive cytokinin and 56-hour exposure to inductive auxin for differentiation at 72 hours of culture. Freshly isolated Zinnia cells can be maintained in medium having low concentrations of both auxin and cytokinin for only 1 day without significant loss of potential to differentiate upon transfer to inductive medium. Initial culture for up to 2 days in medium having high auxin and low cytokinin, or low auxin and high cytokinin, allows full differentiation on the third day after transfer to inductive medium and potentiates the early differentiation of some cells.
Document ID
20040112089
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Church, D. L.
(Yale University New Haven, Connecticut 06511, United States)
Galston, A. W.
Date Acquired
August 21, 2013
Publication Date
January 1, 1988
Publication Information
Publication: Plant physiology
Volume: 88
ISSN: 0032-0889
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: 5-T32-GM07223-12
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Program Space Biology
NASA Discipline Plant Biology
NASA Discipline Number 40-10

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