NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Production and characterization of monoclonal antibodies to wall-localized peroxidases from corn seedlingsA library of 22 hybridomas, which make antibodies to soluble wall antigens from the coleoptiles and primary leaves of etiolated corn (Zea mays L.) seedlings, was raised and cloned three times by limit dilution to assure monoclonal growth and stability. Two of these hybridomas made immunoglobulin G antibodies, designated mWP3 and mWP19, which both effectively immunoprecipitated peroxidase activity from crude and partially purified preparations of wall peroxidases. Direct peroxidase-binding assays revealed that both antibodies bound enzymes with peroxidase activity. As judged by immunoblot analyses, mWP3 recognized a Mr 98,000 wall peroxidase with an isoelectric point near 4.2, and mWP19 recognized a Mr 58,000 wall peroxidase. Immunogold localization studies showed both peroxidases are predominately in cell walls.
Document ID
20040112095
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Kim, S. H.
(University of Texas at Austin 78713 United States)
Terry, M. E.
Hoops, P.
Dauwalder, M.
Roux, S. J.
Date Acquired
August 21, 2013
Publication Date
January 1, 1988
Publication Information
Publication: Plant physiology
Volume: 88
ISSN: 0032-0889
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: NSG 7480
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 40-10
NASA Discipline Plant Biology
Non-NASA Center
NASA Program Space Biology

Available Downloads

There are no available downloads for this record.
No Preview Available