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Polymer mobility in cell walls of cucumber hypocotylsCell walls were prepared from the growing region of cucumber (Cucumis sativus) hypocotyls and examined by solid-state 13C NMR spectroscopy, in both enzymically active and inactivated states. The rigidity of individual polymer segments within the hydrated cell walls was assessed from the proton magnetic relaxation parameter, T2, and from the kinetics of cross-polarisation from 1H to 13C. The microfibrils, including most of the xyloglucan in the cell wall, as well as cellulose, behaved as very rigid solids. A minor xyloglucan fraction, which may correspond to cross-links between microfibrils, shared a lower level of rigidity with some of the pectic galacturonan. Other pectins, including most of the galactan side-chain residues of rhamnogalacturonan I, were much more mobile and behaved in a manner intermediate between the solid and liquid states. The only difference observed between the enzymically active and inactive cell walls, was the loss of a highly mobile, methyl-esterified galacturonan fraction, as the result of pectinesterase activity.
Document ID
20040112178
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Fenwick, K. M.
(Glasgow University United Kingdom)
Apperley, D. C.
Cosgrove, D. J.
Jarvis, M. C.
Date Acquired
August 21, 2013
Publication Date
May 1, 1999
Publication Information
Publication: Phytochemistry
Volume: 51
Issue: 1
ISSN: 0031-9422
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Plant Biology
Non-NASA Center

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