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Complex polar lipids of a hot spring cyanobacterial mat and its cultivated inhabitantsThe complex polar lipids of the hot spring cyanobacterial mat in the 50 to 55 degrees C region of Octopus Spring, Yellowstone National Park, and of thermophilic bacteria cultivated from this or similar habitats, were compared in an attempt to understand the microbial sources of the major lipid biomarkers in this community. Intact complex lipids were analyzed directly by fast atom bombardment mass spectrometry (FAB-MS), two-dimensional thin-layer chromatography (TLC), and combined TLC-FAB-MS. FAB-MS and TLC gave qualitatively similar results, suggesting that the mat contains major lipids most like those of the cyanobacterial isolate we studied, Synechococcus sp. strain Y-7c-s. These include monoglycosyl, diglycosyl, and sulfoquinosovyl diglycerides (MG, DG, and SQ, respectively) and phosphatidyl glycerol (PG). Though Chloroflexus aurantiacus also contains MG, DG, and PG, the fatty acid chain lengths of mat MGs, DGs, and PGs resemble more those of cyanobacterial than green nonsulfur bacterial lipids. FAB-MS spectra of the lipids of nonphototrophic bacterial isolates were distinctively different from those of the mat and phototrophic isolates. The lipids of these nonphototrophic isolates were not detected in the mat, but most could be detected when added to mat samples. The mat also contains major glycolipids and aminophospholipids of unknown structure and origin. FAB-MS and TLC did not always give quantitatively similar results. In particular, PG and SQ may give disproportionately high FAB-MS responses.
Document ID
20040112237
Acquisition Source
Headquarters
Document Type
Reprint (Version printed in journal)
Authors
Ward, D. M.
(Montana State University Bozeman 59717, United States)
Panke, S.
Kloppel, K. D.
Christ, R.
Fredrickson, H.
Date Acquired
August 21, 2013
Publication Date
September 1, 1994
Publication Information
Publication: Applied and environmental microbiology
Volume: 60
Issue: 9
ISSN: 0099-2240
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: BEO
CONTRACT_GRANT: NAGW-2764
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Exobiology

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