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Record 1 of 523
Comparative assessment of the polypeptide profiles from lateral and primary roots of Phaseolus vulgaris L
External Online Source: doi:10.1002/jez.1402690307
Author and Affiliation:
Westberg, J.(Kansas State University, Division of Biology, Manhattan 66506-4903, United States)
Odom, W. R.
Guikema, J. A.
Spooner, B. S. [Principal Investigator]
Abstract: In Phaseolus vulgaris, primary roots show gravitational sensitivity soon after emerging from the seed. In contrast, lateral roots are agravitropic during early development, and become gravitropic after several cm growth. Primary and lateral root tissues were examined by polyacrylamide gel electrophoresis, coupled with western blotting techniques, to compare proteins which may contribute to the acquisition of gravitational sensitivity. Root tips and zones of cell elongation were compared for each root type, using immunological probes for calmodulin, alpha-actin, alpha-tubulin, and proteins of the plastid envelope. Lateral roots contained qualitatively less calmodulin, and showed a slightly different pattern of actin-related epitope proteins, than did primary root tissues, suggesting that polypeptide differences may contribute to the gravitational sensitivity which these root types express.
Publication Date: Jul 01, 1994
Document ID:
20040112243
(Acquired Oct 05, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: The Journal of experimental zoology (ISSN 0022-104X); Volume 269; 3; 223-9
Publisher Information: United States
Contract/Grant/Task Num: 9337306-9147; NAGW-1197; NAGW-2328
Financial Sponsor: NASA; Washington DC United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: GRAVITY PERCEPTION; LEGUMINOUS PLANTS; PEPTIDES; PLANT ROOTS; PLANTS (BOTANY); POLYPEPTIDES; PROTEINS; CALMODULIN; CYTOLOGY; GRAVITROPISM; MUSCLE FIBERS; PLASTIDS
Other Descriptors: FABACEAE/CHEMISTRY/GROWTH & DEVELOPMENT/PHYSIOLOGY; GRAVITY PERCEPTION/PHYSIOLOGY; PEPTIDES/ANALYSIS/PHYSIOLOGY; PLANT PROTEINS/ANALYSIS/PHYSIOLOGY; PLANT ROOTS/CHEMISTRY/GROWTH & DEVELOPMENT/PHYSIOLOGY; PLANTS, MEDICINAL; ACTINS/ANALYSIS; CALMODULIN/ANALYSIS; COMPARATIVE STUDY; GRAVITROPISM/PHYSIOLOGY; PLANT ROOT CAP/CHEMISTRY/CYTOLOGY/PHYSIOLOGY; PLASTIDS/CHEMISTRY/PHYSIOLOGY; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; TUBULIN/ANALYSIS; NASA DISCIPLINE CELL BIOLOGY; NASA DISCIPLINE DEVELOPMENTAL BIOLOGY; NASA DISCIPLINE NUMBER 93-10; NASA PROGRAM NSCORT; NON-NASA CENTER
Availability Source: Other Sources
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