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Record 42 of 525
Structural analysis of photosystem I polypeptides using chemical crosslinking
External Online Source: doi:10.1002/jez.1402690305
Author and Affiliation:
Armbrust, T. S.(Kansas State University, Division of Biology, Manhattan 66506-4901, United States)
Odom, W. R.
Guikema, J. A.
Spooner, B. S. [Principal Investigator]
Abstract: Thylakoid membranes, obtained from leaves of 14 d soybean (Glycine max L. cv. Williams) plants, were treated with the chemical crosslinkers glutaraldehyde or 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) to investigate the structural organization of photosystem I. Polypeptides were resolved using lithium dodecyl sulfate polyacrylamide gel electrophoresis, and were identified by western blot analysis using a library of polyclonal antibodies specific for photosystem I subunits. An electrophoretic examination of crosslinked thylakoids revealed numerous crosslinked products, using either glutaraldehyde or EDC. However, only a few of these could be identified by western blot analysis using subunit-specific polyclonal antibodies. Several glutaraldehyde dependent crosslinked species were identified. A single band was identified minimally composed of PsaC and PsaD, documenting the close interaction between these two subunits. The most interesting aspect of these studies was a crosslinked species composed of the PsaB subunit observed following EDC treatment of thylakoids. This is either an internally crosslinked species, which will provide structural information concerning the topology of the complex PsaB protein, a linkage with a polypeptide for which we do not yet have an immunological probe, or a masking of epitopes by the EDC linkage at critical locations in the peptide which is linked to PsaB.
Publication Date: Jul 01, 1994
Document ID:
20040112244
(Acquired Oct 05, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: The Journal of experimental zoology; p. 205-11; (ISSN 0022-104X); Volume 269; 3
Publisher Information: United States
Contract/Grant/Task Num: 93-01566; NAGW-1197; NAGW-2328
Financial Sponsor: NASA; Washington DC United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: CHLOROPLASTS; CROSSLINKING; METABOLISM; PHOTOSYNTHESIS; PLANTS (BOTANY); POLYPEPTIDES; PROTEINS; SOYBEANS; STRUCTURAL ANALYSIS; LEAVES; REAGENTS
Other Descriptors: CHLOROPLASTS/METABOLISM/ULTRASTRUCTURE; MEMBRANE PROTEINS/ANALYSIS/CHEMISTRY/METABOLISM; PHOTOSYNTHETIC REACTION CENTER COMPLEX PROTEINS/ANALYSIS/CHEMISTRY/METABOLISM; PLANT PROTEINS/ANALYSIS/CHEMISTRY/METABOLISM; SOYBEANS/CHEMISTRY/METABOLISM/ULTRASTRUCTURE; CROSS-LINKING REAGENTS; PHOTOSYNTHESIS/PHYSIOLOGY; PHOTOSYSTEM I PROTEIN COMPLEX; PLANT LEAVES/CHEMISTRY/METABOLISM/ULTRASTRUCTURE; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; NASA DISCIPLINE CELL BIOLOGY; NASA DISCIPLINE DEVELOPMENTAL BIOLOGY; NASA DISCIPLINE NUMBER 93-10; NASA PROGRAM NSCORT; NON-NASA CENTER
Availability Source: Other Sources
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