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Evidence for a role in growth and salt resistance of a plasma membrane H+-ATPase in the root endodermisThe plasma membrane of plant cells is energized by an electrochemical gradient produced by P-type H+-ATPases (proton pumps). These pumps are encoded by at least 12 genes in Arabidopsis. Here we provide evidence that isoform AHA4 contributes to solute transport through the root endodermis. AHA4 is expressed most strongly in the root endodermis and flowers, as suggested by promoter-GUS reporter assays. A disruption of this pump (aha4-1) was identified as a T-DNA insertion in the middle of the gene (after VFP(574)). Truncated aha4-1 transcripts accumulate to approximately 50% of the level observed for AHA4 mRNA in wild-type plants. Plants homozygous for aha4-1 (-/-) show a subtle reduction in root and shoot growth compared with wild-type plants when grown under normal conditions. However, a mutant phenotype is very clear in plants grown under salt stress (e.g., 75 or 110 mM NaCl). In leaves of mutant plants subjected to Na stress, the ratio of Na to K increased 4-5-fold. Interestingly, the aha4-1 mutation appears to be semidominant and was only partially complemented by the introduction of additional wild-type copies of AHA4. These results are consistent with the hypothesis that aha4-1 may produce a dominant negative protein or RNA that partially disrupts the activity of other pumps or functions in the root endodermal tissue, thereby compromising the function of this cell layer in controlling ion homeostasis and nutrient transport.
Document ID
Document Type
Reprint (Version printed in journal)
Vitart, V. (The Scripps Research Institute La Jolla, CA 92037, United States)
Baxter, I.
Doerner, P.
Harper, J. F.
Evans, M. L.
Date Acquired
August 21, 2013
Publication Date
August 1, 2001
Publication Information
Publication: The Plant journal : for cell and molecular biology
Volume: 27
Issue: 3
ISSN: 0960-7412
Subject Category
Life Sciences (General)
Distribution Limits
NASA Discipline Plant Biology
Non-NASA Center