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Hindlimb unweighting decreases endothelium-dependent dilation and eNOS expression in soleus not gastrocnemiusWe tested the hypothesis that hindlimb unweighting (HLU) decreases endothelium-dependent vasodilation and expression of endothelial nitric oxide synthase (eNOS) and superoxide dismutase-1 (SOD-1) in arteries of skeletal muscle with reduced blood flow during HLU. Sprague-Dawley rats (300-350 g) were exposed to HLU (n = 15) or control (n = 15) conditions for 14 days. ACh-induced dilation was assessed in muscle with reduced [soleus (Sol)] or unchanged [gastrocnemius (Gast)] blood flow during HLU. eNOS and SOD-1 expression were measured in feed arteries (FA) and in first-order (1A), second-order (2A), and third-order (3A) arterioles. Dilation to infusion of ACh in vivo was blunted in Sol but not Gast. In arteries of Sol muscle, HLU decreased eNOS mRNA and protein content. eNOS mRNA content was significantly less in Sol FA (35%), 1A arterioles (25%) and 2A arterioles (18%). eNOS protein content was less in Sol FA (64%) and 1A arterioles (65%) from HLU rats. In arteries of Gast, HLU did not decrease eNOS mRNA or protein. SOD-1 mRNA expression was less in Sol 2A arterioles (31%) and 3A arterioles (29%) of HLU rats. SOD-1 protein content was less in Sol FA (67%) but not arterioles. SOD-1 mRNA and protein content were not decreased in arteries from Gast. These data indicate that HLU decreases endothelium-dependent vasodilation, eNOS expression, and SOD-1 expression primarily in arteries of Sol muscle where blood flow is reduced during HLU.
Document ID
20040112323
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Woodman, C. R.
(University of Missouri Columbia, Missouri 65211, United States)
Schrage, W. G.
Rush, J. W.
Ray, C. A.
Price, E. M.
Hasser, E. M.
Laughlin, M. H.
Date Acquired
August 21, 2013
Publication Date
September 1, 2001
Publication Information
Publication: Journal of applied physiology (Bethesda, Md. : 1985)
Volume: 91
Issue: 3
ISSN: 8750-7587
Subject Category
Aerospace Medicine
Funding Number(s)
CONTRACT_GRANT: HL-52490
CONTRACT_GRANT: HL-09739
CONTRACT_GRANT: HL-36088
CONTRACT_GRANT: HL-55306
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Cardiopulmonary
Non-NASA Center

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