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Antigen S1, encoded by the MIC1 gene, is characterized as an epitope of human CD59, enabling measurement of mutagen-induced intragenic deletions in the AL cell systemS1 cell membrane antigen is encoded by the MIC1 gene on human chromosome 11. This antigen has been widely used as a marker for studies in gene mapping or in analysis of mutagen-induced gene deletions/mutations, which utilized the human-hamster hybrid cell-line, AL-J1, carrying human chromosome 11. Evidence is presented here which identifies S1 as an epitope of CD59, a cell membrane complement inhibiting protein. E7.1 monoclonal antibody, specific for the S1 determinant, was found to react strongly with membrane CD59 in Western blotting, and to bind to purified, urinary form of CD59 in ELISAs. Cell membrane expression of S1 on various cell lines always correlated with that of CD59 when examined by immunofluorescent staining. In addition, E7.1 antibody inhibited the complement regulatory function of CD59. Identification of S1 protein as CD59 has increased the scope of the AL cell system by enabling analysis of intragenic mutations, and multiplex PCR analysis of mutated cells is described, showing variable loss of CD59 exons.
Document ID
20040112377
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Wilson, A. B.
(University of Cambridge United Kingdom)
Seilly, D.
Willers, C.
Vannais, D. B.
McGraw, M.
Waldren, C. A.
Hei, T. K.
Davies, A.
Chatterjee, A.
Date Acquired
August 21, 2013
Publication Date
May 1, 1999
Publication Information
Publication: Somatic cell and molecular genetics
Volume: 25
Issue: 3
ISSN: 0740-7750
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: 5T32CA09236
CONTRACT_GRANT: CA36447
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Radiation Health

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