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Flavonoid accumulation patterns of transparent testa mutants of arabidopsisFlavonoids have been implicated in the regulation of auxin movements in Arabidopsis. To understand when and where flavonoids may be acting to control auxin movement, the flavonoid accumulation pattern was examined in young seedlings and mature tissues of wild-type Arabidopsis. Using a variety of biochemical and visualization techniques, flavonoid accumulation in mature plants was localized in cauline leaves, pollen, stigmata, and floral primordia, and in the stems of young, actively growing inflorescences. In young Landsberg erecta seedlings, aglycone flavonols accumulated developmentally in three regions, the cotyledonary node, the hypocotyl-root transition zone, and the root tip. Aglycone flavonols accumulated at the hypocotyl-root transition zone in a developmental and tissue-specific manner with kaempferol in the epidermis and quercetin in the cortex. Quercetin localized subcellularly in the nuclear region, plasma membrane, and endomembrane system, whereas kaempferol localized in the nuclear region and plasma membrane. The flavonoid accumulation pattern was also examined in transparent testa mutants blocked at different steps in the flavonoid biosynthesis pathway. The transparent testa mutants were shown to have precursor accumulation patterns similar to those of end product flavonoids in wild-type Landsberg erecta, suggesting that synthesis and end product accumulation occur in the same cells.
Document ID
Document Type
Reprint (Version printed in journal)
External Source(s)
Peer, W. A. (Purdue University West Lafayette, Indiana 47907, United States)
Brown, D. E.
Tague, B. W.
Muday, G. K.
Taiz, L.
Murphy, A. S.
Date Acquired
August 21, 2013
Publication Date
June 1, 2001
Publication Information
Publication: Plant physiology
Volume: 126
Issue: 2
ISSN: 0032-0889
Subject Category
Life Sciences (General)
Distribution Limits
Non-NASA Center
NASA Discipline Plant Biology