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Vitamin C-sulfate inhibits mineralization in chondrocyte cultures: a caveatDifferentiating chick limb-bud mesenchymal cell micro-mass cultures routinely mineralize in the presence of 10% fetal calf serum, antibiotics, 4 mM inorganic phosphate (or 2.5 mM beta-glycerophosphate), 0.3 mg/ml glutamine and either 25 microg/ml vitamin C or 5-12 microg/ml vitamin C-sulfate. The failure of these cultures to produce a mineralized matrix (assessed by electron microscopy, 45Ca uptake and Fourier transform infrared microscopy) led to the evaluation of each of these additives. We report here that the "stable" vitamin C-sulfate (ascorbic acid-2-sulfate) causes increased sulfate incorporation into the cartilage matrix. Furthermore, the release of sulfate from the vitamin C derivative appears to be responsible for the inhibition of mineral deposition, as demonstrated in cultures with equimolar amounts of vitamin C and sodium sulfate.
Document ID
20040112460
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Boskey, A. L.
(The Hospital for Special Surgery 535 East 70th Street, New York, NY 10021, United States)
Blank, R. D.
Doty, S. B.
Date Acquired
August 21, 2013
Publication Date
April 1, 2001
Publication Information
Publication: Matrix biology : journal of the International Society for Matrix Biology
Volume: 20
Issue: 2
ISSN: 0945-053X
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: AR037661A
CONTRACT_GRANT: AR 46121
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Musculoskeletal
Non-NASA Center

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