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Cell-cycle research with synchronous cultures: an evaluationThe baby-machine system, which produces new-born Escherichia coli cells from cultures immobilized on a membrane, was developed many years ago in an attempt to attain optimal synchrony with minimal disturbance of steady-state growth. In the present article, we put forward a model to describe the behaviour of cells produced by this method, and provide quantitative evaluation of the parameters involved, at each of four different growth rates. Considering the high level of selection achievable with this technique and the natural dispersion in interdivision times, we believe that the output of the baby machine is probably close to optimal in terms of both quality and persistence of synchrony. We show that considerable information on events in the cell cycle can be obtained from populations with age distributions very much broader than those achieved with the baby machine and differing only modestly from steady state. The data presented here, together with the long and fruitful history of findings employing the baby-machine technique, suggest that minimisation of stress on cells is the single most important factor for successful cell-cycle analysis.
Document ID
20040112517
Document Type
Reprint (Version printed in journal)
Authors
Helmstetter, C. E. (Florida Institute of Technology Melbourne 32901, United States)
Thornton, M.
Grover, N. B.
Date Acquired
August 21, 2013
Publication Date
January 1, 2001
Publication Information
Publication: Biochimie
Volume: 83
Issue: 1
ISSN: 0300-9084
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology