NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
High- and low-LET induced chromosome damage in human lymphocytes: a time-course of aberrations in metaphase and interphasePURPOSE: To investigate how cell-cycle delays in human peripheral lymphocytes affect the expression of complex chromosome damage in metaphase following high- and low-LET radiation exposure. MATERIALS AND METHODS: Whole blood was irradiated in vitro with a low and a high dose of 1 GeV u(-1) iron particles, 400MeV u(-1) neon particles or y-rays. Lymphocytes were cultured and metaphase cells were collected at different time points after 48-84h in culture. Interphase chromosomes were prematurely condensed using calyculin-A, either 48 or 72 h after exposure to iron particles or gamma-rays. Cells in first division were analysed using a combination of FISH whole-chromosome painting and DAPI/ Hoechst 33258 harlequin staining. RESULTS: There was a delay in expression of chromosome damage in metaphase that was LET- and dose-dependant. This delay was mostly related to the late emergence of complex-type damage into metaphase. Yields of damage in PCC collected 48 h after irradiation with iron particles were similar to values obtained from cells undergoing mitosis after prolonged incubation. CONCLUSION: The yield of high-LET radiation-induced complex chromosome damage could be underestimated when analysing metaphase cells collected at one time point after irradiation. Chemically induced PCC is a more accurate technique since problems with complicated cell-cycle delays are avoided.
Document ID
20040112535
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
George, K.
(NASA Johnson Space Center Houston TX United States)
Wu, H.
Willingham, V.
Furusawa, Y.
Kawata, T.
Cucinotta, F. A.
Dicello, J. F.
Date Acquired
August 21, 2013
Publication Date
February 1, 2001
Publication Information
Publication: International journal of radiation biology
Volume: 77
Issue: 2
ISSN: 0955-3002
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Radiation Health

Available Downloads

There are no available downloads for this record.
No Preview Available