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A role for chromosomal instability in the development of and selection for radioresistant cell variantsChromosome instability is a common occurrence in tumour cells. We examined the hypothesis that the elevated rate of mutation formation in unstable cells can lead to the development of clones of cells that are resistant to the cancer therapy. To test this hypothesis, we compared chromosome instability to radiation sensitivity in 30 independently isolated clones of GM10115 human-hamster hybrid cells. There was a broader distribution of radiosensitivity and a higher mean SF(2)in chromosomally unstable clones. Cytogenetic and DNA double-strand break rejoining assays suggest that sensitivity was a function of DNA repair efficiency. In the unstable population, the more radioresistant clones also had significantly lower plating efficiencies. These observations suggest that chromosome instability in GM10115 cells can lead to the development of cell variants that are more resistant to radiation. In addition, these results suggest that the process of chromosome breakage and recombination that accompanies chromosome instability might provide some selective pressure for more radioresistant variants. Copyright 2001 Cancer Research Campaign.
Document ID
20040112574
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Limoli, C. L.
(University of California San Francisco, CA 94103-0806, United States)
Corcoran, J. J.
Jordan, R.
Morgan, W. F.
Schwartz, J. L.
Date Acquired
August 21, 2013
Publication Date
February 1, 2001
Publication Information
Publication: British journal of cancer
Volume: 84
Issue: 4
ISSN: 0007-0920
Subject Category
Aerospace Medicine
Funding Number(s)
CONTRACT_GRANT: CA 73931
CONTRACT_GRANT: CA 73924
CONTRACT_GRANT: GM 54189
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Radiation Health
Non-NASA Center

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