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Comparison of GLUT1, GLUT3, and GLUT4 mRNA and the subcellular distribution of their proteins in normal human muscleBasal, "insulin-independent" glucose uptake into skeletal muscle is provided by glucose transporters positioned at the plasma membrane. The relative amount of the three glucose transporters expressed in muscle has not been previously quantified. Using a combination of qualitative and quantitative ribonuclease protection assay (RPA) methods, we found in normal human muscle that GLUT1, GLUT3, and GLUT4 mRNA were expressed at 90 +/- 10, 46 +/- 4, and 156 +/- 12 copies/ng RNA, respectively. Muscle was fractionated by DNase digestion and differential sedimentation into membrane fractions enriched in plasma membranes (PM) or low-density microsomes (LDM). GLUT1 and GLUT4 proteins were distributed 57% to 67% in LDM, whereas GLUT3 protein was at least 88% in the PM-enriched fractions. These data suggest that basal glucose uptake into resting human muscle could be provided in part by each of these three isoforms.
Document ID
20040112631
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Stuart, C. A. (The University of Texas Medical Branch at Galveston 77555-1060, United States)
Wen, G.
Gustafson, W. C.
Thompson, E. A.
Date Acquired
August 21, 2013
Publication Date
December 1, 2000
Publication Information
Publication: Metabolism: clinical and experimental
Volume: 49
Issue: 12
ISSN: 0026-0495
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: DK33749
CONTRACT_GRANT: MO1 RR00073
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Regulatory Physiology
Non-NASA Center