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Record 5 of 1212
Gene amplification and microsatellite instability induced in tumorigenic human bronchial epithelial cells by alpha particles and heavy ions
Author and Affiliation:
Piao, C. Q.(College of Physicians & Surgeons, Columbia University, Center for Radiological Research, New York, New York 10032, United States)
Hei, T. K.
Hall, E. J. [Principal Investigator]
Abstract: Gene amplification and microsatellite alteration are useful markers of genomic instability in tumor and transformed cell lines. It has been suggested that genomic instability contributes to the progression of tumorigenesis by accumulating genetic changes. In this study, amplification of the carbamyl-P-synthetase, aspartate transcarbamylase, dihydro-orotase (CAD) gene in transformed and tumorigenic human bronchial epithelial (BEP2D) cells induced by either alpha particles or (56)Fe ions was assessed by measuring resistance to N-(phosphonacetyl)-l-aspartate (PALA). In addition, alterations of microsatellite loci located on chromosomes 3p and 18q were analyzed in a series of primary and secondary tumor cell lines generated in nude mice. The frequency of PALA-resistant colonies was 1-3 x 10(-3) in tumor cell lines, 5-8 x 10(-5) in transformed cells prior to inoculation into nude mice, and less than 10(-7) in control BEP2D cells. Microsatellite alterations were detected in all 11 tumor cell lines examined at the following loci: D18S34, D18S363, D18S877, D3S1038 and D3S1607. No significant difference in either PALA resistance or microsatellite instability was found in tumor cell lines that were induced by alpha particles compared to those induced by (56)Fe ions.
Publication Date: Jan 01, 2001
Document ID:
20040112654
(Acquired Oct 05, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Radiation research (ISSN 0033-7587); Volume 155; 1 Pt 2; 263-267
Publisher Information: United States
Contract/Grant/Task Num: CA-49062; ES-07890; RR 11523
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ALPHA PARTICLES; AMPLIFICATION; ASPARTIC ACID; BRONCHI; GENE EXPRESSION REGULATION; GENETICS; HEAVY IONS; LUNGS; MICROSATELLITES; NEOPLASMS; RADIATION EFFECTS; TUMORS; CHROMOSOMES; CULTURED CELLS; ENZYMES; EPITHELIUM; IRON; MICE; PROTEINS
Other Descriptors: ALPHA PARTICLES; ASPARTIC ACID/ANALOGS & DERIVATIVES/PHARMACOLOGY; BRONCHI/PHYSIOLOGY/RADIATION EFFECTS/ULTRASTRUCTURE; CELL TRANSFORMATION, NEOPLASTIC/GENETICS/RADIATION EFFECTS; GENE AMPLIFICATION/RADIATION EFFECTS; HEAVY IONS; LUNG NEOPLASMS/GENETICS; NEOPLASMS, RADIATION-INDUCED/GENETICS; PHOSPHONOACETIC ACID/ANALOGS & DERIVATIVES/PHARMACOLOGY; ANIMALS; ANTIMETABOLITES, ANTINEOPLASTIC/PHARMACOLOGY; ASPARTATE CARBAMOYLTRANSFERASE/GENETICS; CARBAMOYL-PHOSPHATE SYNTHASE (GLUTAMINE-HYDROLYZING)/GENETICS; CHROMOSOMES, HUMAN, PAIR 18/RADIATION EFFECTS; CHROMOSOMES, HUMAN, PAIR 3/RADIATION EFFECTS; DIHYDROOROTASE/GENETICS; DRUG RESISTANCE, NEOPLASM; EPITHELIAL CELLS/PHYSIOLOGY/RADIATION EFFECTS/ULTRASTRUCTURE; HUMAN; IRON; MICE; MICE, NUDE; MICROSATELLITE REPEATS; MULTIENZYME COMPLEXES/GENETICS; NEOPLASM PROTEINS/GENETICS; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; TUMOR CELLS, CULTURED/DRUG EFFECTS; XENOGRAFT MODEL ANTITUMOR ASSAYS; NASA DISCIPLINE RADIATION HEALTH; NON-NASA CENTER
Availability Source: Other Sources
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