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Bioenergetics and mitochondrial transmembrane potential during differentiation of cultured osteoblastsTo evaluate the relationship between osteoblast differentiation and bioenergetics, cultured primary osteoblasts from fetal rat calvaria were grown in medium supplemented with ascorbate to induce differentiation. Before ascorbate treatment, the rate of glucose consumption was 320 nmol. h(-1). 10(6) cells(-1), respiration was 40 nmol. h(-1). 10(6) cells(-1), and the ratio of lactate production to glucose consumption was approximately 2, indicating that glycolysis was the main energy source for immature osteoblasts. Ascorbate treatment for 14 days led to a fourfold increase in respiration, a threefold increase in ATP production, and a fivefold increase in ATP content compared with that shown in immature cells. Confocal imaging of mitochondria stained with a transmembrane potential-sensitive vital dye showed that mature cells possessed abundant amounts of high-transmembrane-potential mitochondria, which were concentrated near the culture medium-facing surface. Acute treatment of mature osteoblasts with metabolic inhibitors showed that the rate of glycolysis rose to maintain the cellular energy supply constant. Thus progressive differentiation coincided with changes in cellular metabolism and mitochondrial activity, which are likely to play key roles in osteoblast function.
Document ID
20040112745
Acquisition Source
Ames Research Center
Document Type
Reprint (Version printed in journal)
Authors
Komarova, S. V.
(NASA Ames Research Center Moffett Field CA United States)
Ataullakhanov, F. I.
Globus, R. K.
Date Acquired
August 21, 2013
Publication Date
October 1, 2000
Publication Information
Publication: American journal of physiology. Cell physiology
Volume: 279
Issue: 4
ISSN: 0363-6143
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Center ARC
NASA Discipline Musculoskeletal

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