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Cell culture for three-dimensional modeling in rotating-wall vessels: an application of simulated microgravityHigh-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 X 10(7) cells/ml. Cells on microcarriers were observed to grow with enhanced bridging in this batch culture system. The RWV is a horizontally rotated tissue culture vessel with silicon membrane oxygenation. This design results in a low-turbulence, low-shear cell culture environment with abundant oxygenation. The RWV has the potential to culture a wide variety of normal and neoplastic cells.
Document ID
20040120687
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Schwarz, R. P. (KRUG Life Sciences Houston, Texas 77058, United States)
Goodwin, T. J.
Wolf, D. A.
Date Acquired
August 22, 2013
Publication Date
January 1, 1992
Publication Information
Publication: Journal of tissue culture methods : Tissue Culture Association manual of cell, tissue, and organ culture procedures
Volume: 14
Issue: 2
ISSN: 0271-8057
Subject Category
Aerospace Medicine
Funding Number(s)
CONTRACT_GRANT: NAS9-17720
Distribution Limits
Public
Copyright
Other