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Peritoneal culture alters Streptococcus pneumoniae protein profiles and virulence propertiesWe have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial cell lines at levels 10-fold greater than those cultured in vitro.
Document ID
20040141401
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Orihuela, C. J.
(University of Texas Medical Branch Galveston, Texas 77555-1019, United States)
Janssen, R.
Robb, C. W.
Watson, D. A.
Niesel, D. W.
Date Acquired
August 22, 2013
Publication Date
October 1, 2000
Publication Information
Publication: Infection and immunity
Volume: 68
Issue: 10
ISSN: 0019-9567
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Environmental Health

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