NASA Logo, External Link
Facebook icon, External Link to NASA STI page on Facebook Twitter icon, External Link to NASA STI on Twitter YouTube icon, External Link to NASA STI Channel on YouTube RSS icon, External Link to New NASA STI RSS Feed AddThis share icon
 

Record Details

Record 1 of 106
Distinct abscisic acid signaling pathways for modulation of guard cell versus mesophyll cell potassium channels revealed by expression studies in Xenopus laevis oocytes
External Online Source: doi:10.1104/pp.124.1.223
Author and Affiliation:
Sutton, F.(South Dakota State University, Plant Science Department, Box 2108, Brookings, South Dakota 57007, United States)
Paul, S. S.
Wang, X. Q.
Assmann, S. M.
Evans, M. L. [Principal Investigator]
Abstract: Regulation of guard cell ion transport by abscisic acid (ABA) and in particular ABA inhibition of a guard cell inward K(+) current (I(Kin)) is well documented. However, little is known concerning ABA effects on ion transport in other plant cell types. Here we applied patch clamp techniques to mesophyll cell protoplasts of fava bean (Vicia faba cv Long Pod) plants and demonstrated ABA inhibition of an outward K(+) current (I(Kout)). When mesophyll cell protoplast mRNA (mesophyll mRNA) was expressed in Xenopus laevis oocytes, I(Kout) was generated that displayed similar properties to I(Kout) observed from direct analysis of mesophyll cell protoplasts. I(Kout) expressed by mesophyll mRNA-injected oocytes was inhibited by ABA, indicating that the ABA signal transduction pathway observed in mesophyll cells was preserved in the frog oocytes. Co-injection of oocytes with guard cell protoplast mRNA and cRNA for KAT1, an inward K(+) channel expressed in guard cells, resulted in I(Kin) that was similarly inhibited by ABA. However, oocytes co-injected with mesophyll mRNA and KAT1 cRNA produced I(Kin) that was not inhibited by ABA. These results demonstrate that the mesophyll-encoded signaling mechanism could not substitute for the guard cell pathway. These findings indicate that mesophyll cells and guard cells use distinct and different receptor types and/or signal transduction pathways in ABA regulation of K(+) channels.
Publication Date: Sep 01, 2000
Document ID:
20040141406
(Acquired Nov 09, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Plant physiology (ISSN 0032-0889); Volume 124; 1; 223-30
Publisher Information: United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ABSCISIC ACID; GAMETOCYTES; ION CHANNELS (BIOLOGY); LEAVES; LEGUMINOUS PLANTS; METABOLISM; MODULATION; PLANTS (BOTANY); POTASSIUM; PROTEINS; SIGNAL TRANSMISSION; AMPHIBIA; CYTOLOGY; GENETICS; RIBONUCLEIC ACIDS
Other Descriptors: ABSCISIC ACID/METABOLISM; FABACEAE/CYTOLOGY/METABOLISM/PHYSIOLOGY; PLANT LEAVES/CYTOLOGY/METABOLISM/PHYSIOLOGY; PLANT PROTEINS/ANTAGONISTS & INHIBITORS/METABOLISM/PHYSIOLOGY; PLANTS, MEDICINAL; POTASSIUM CHANNELS/GENETICS/METABOLISM/PHYSIOLOGY; SIGNAL TRANSDUCTION; ANIMALS; IN VITRO; OOCYTES/METABOLISM/PHYSIOLOGY; PATCH-CLAMP TECHNIQUES; POTASSIUM CHANNEL BLOCKERS; RNA, MESSENGER/GENETICS/METABOLISM; SUPPORT, NON-U.S. GOV'T; XENOPUS LAEVIS; NASA DISCIPLINE PLANT BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
› Back to Top
NASA Logo, External Link
NASA Official: Gerald Steeman
Site Curator: STI Program
Last Modified: August 23, 2011
Contact Us