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Record Details

Record 4 of 2244
Arabidopsis chloroplast chaperonin 10 is a calmodulin-binding protein
External Online Source: doi:10.1006/bbrc.2000.3335
Author and Affiliation:
Yang, T.(Washington State University, Laboratory of Plant Molecular Biology and Physiology, Pullman, Washington 99164, United States)
Poovaiah, B. W.
Abstract: Calcium regulates diverse cellular activities in plants through the action of calmodulin (CaM). By using (35)S-labeled CaM to screen an Arabidopsis seedling cDNA expression library, a cDNA designated as AtCh-CPN10 (Arabidopsis thaliana chloroplast chaperonin 10) was cloned. Chloroplast CPN10, a nuclear-encoded protein, is a functional homolog of E. coli GroES. It is believed that CPN60 and CPN10 are involved in the assembly of Rubisco, a key enzyme involved in the photosynthetic pathway. Northern analysis revealed that AtCh-CPN10 is highly expressed in green tissues. The recombinant AtCh-CPN10 binds to CaM in a calcium-dependent manner. Deletion mutants revealed that there is only one CaM-binding site in the last 31 amino acids of the AtCh-CPN10 at the C-terminal end. The CaM-binding region in AtCh-CPN10 has higher homology to other chloroplast CPN10s in comparison to GroES and mitochondrial CPN10s, suggesting that CaM may only bind to chloroplast CPN10s. Furthermore, the results also suggest that the calcium/CaM messenger system is involved in regulating Rubisco assembly in the chloroplast, thereby influencing photosynthesis. Copyright 2000 Academic Press.
Publication Date: Aug 28, 2000
Document ID:
20040141418
(Acquired Nov 09, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Biochemical and biophysical research communications; p. 601-7; (ISSN 0006-291X); Volume 275; 2
Publisher Information: United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: CALMODULIN; CHLOROPLASTS; PLANTS (BOTANY); PROTEINS; AMINO ACIDS; CHEMICAL BONDS; CLONING (BIOLOGY); COMPLEMENTARY DNA; GENE EXPRESSION; GENETIC ENGINEERING; GENETICS; HOMOLOGY
Other Descriptors: ARABIDOPSIS/METABOLISM; CALMODULIN-BINDING PROTEINS/CHEMISTRY/GENETICS/METABOLISM; CHAPERONIN 10/CHEMISTRY/GENETICS/METABOLISM; CHLOROPLASTS/METABOLISM; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; RECOMBINANT PROTEINS/CHEMISTRY/GENETICS/METABOLISM; SEQUENCE HOMOLOGY, AMINO ACID; SUPPORT, U.S. GOV'T, NON-P.H.S; NASA DISCIPLINE PLANT BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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