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Phylogeny of marine Bacillus isolates from the Gulf of MexicoThe phylogeny of 11 pigmented, aerobic, spore-forming isolates from marine sources was studied. Forty-two biochemical characteristics were examined, and a 16S rDNA sequence was obtained for each isolate. In a phylogenetic tree based on 16S sequencing, four isolates (NRRL B-14850, NRRL B-14904, NRRL B-14907, and NRRL B-14908) clustered with B. subtilis and related organisms; NRRL B-14907 was closely related to B. amyloliquefaciens. NRRL B-14907 and NRRL B-14908 were phenotypically similar to B. amyloliquefaciens and B. pumilus, respectively. Three strains (NRRL B-14906, NRRL B-14910, and NRRL B-14911) clustered in a clade that included B. firmus, B. lentus, and B. megaterium. NRRL B-14910 was closely related phenotypically and phylogenetically to B. megaterium. NRRL B-14905 clustered with the mesophilic round spore-producing species, B. fusiformis and B. sphaericus; the isolate was more closely related to B. fusiformis. NRRL B-14905 displayed characteristics typical of the B. sphaericus-like organisms. NRRL B-14909 and NRRL B-14912 clustered with the Paenibacillus species and displayed characteristics typical of the genus. Only NRRL B-14851, an unusually thin rod that forms very small spores, may represent a new Bacillus species.
Document ID
Document Type
Reprint (Version printed in journal)
External Source(s)
Siefert, J. L. (University of Houston TX 77204-5513, United States)
Larios-Sanz, M.
Nakamura, L. K.
Slepecky, R. A.
Paul, J. H.
Moore, E. R.
Fox, G. E.
Jurtshuk, P. Jr
Date Acquired
August 22, 2013
Publication Date
August 1, 2000
Publication Information
Publication: Current microbiology
Volume: 41
Issue: 2
ISSN: 0343-8651
Subject Category
Life Sciences (General)
Funding Number(s)
Distribution Limits
NASA Discipline Exobiology
Non-NASA Center