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Record Details

Record 80 of 1929
Release of kinesin from vesicles by hsc70 and regulation of fast axonal transport
Author and Affiliation:
Tsai, M. Y.(University of Texas Southwestern Medical Center, Department of Cell Biology and Neuroscience, Dallas, Texas 75390-9039, United States)
Morfini, G.
Szebenyi, G.
Brady, S. T.
Abstract: The nature of kinesin interactions with membrane-bound organelles and mechanisms for regulation of kinesin-based motility have both been surprisingly difficult to define. Most kinesin is recovered in supernatants with standard protocols for purification of motor proteins, but kinesin recovered on membrane-bound organelles is tightly bound. Partitioning of kinesin between vesicle and cytosolic fractions is highly sensitive to buffer composition. Addition of either N-ethylmaleimide or EDTA to homogenization buffers significantly increased the fraction of kinesin bound to organelles. Given that an antibody against kinesin light chain tandem repeats also releases kinesin from vesicles, these observations indicated that specific cytoplasmic factors may regulate kinesin release from membranes. Kinesin light tandem repeats contain DnaJ-like motifs, so the effects of hsp70 chaperones were evaluated. Hsc70 released kinesin from vesicles in an MgATP-dependent and N-ethylmaleimide-sensitive manner. Recombinant kinesin light chains inhibited kinesin release by hsc70 and stimulated the hsc70 ATPase. Hsc70 actions may provide a mechanism to regulate kinesin function by releasing kinesin from cargo in specific subcellular domains, thereby effecting delivery of axonally transported materials.
Publication Date: Jun 01, 2000
Document ID:
20040141536
(Acquired Nov 09, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Molecular biology of the cell (ISSN 1059-1524); Volume 11; 6; 2161-73
Publisher Information: United States
Contract/Grant/Task Num: AG-12646; NS-23320; NS-23868
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: AXONS; NERVES; AMINO ACIDS; CELLS (BIOLOGY); CULTURED CELLS; CYTOLOGY; DETERGENTS; HAMSTERS; LUMINESCENT PROTEINS; MICE; ORGANELLES; STEROIDS
Other Descriptors: AXONAL TRANSPORT/PHYSIOLOGY; CARRIER PROTEINS/METABOLISM; KINESIN/ISOLATION & PURIFICATION/METABOLISM; 3T3 CELLS; AMINO ACID SEQUENCE; ANIMALS; CELL LINE; DETERGENTS; DIGITONIN; EDETIC ACID; ETHYLMALEIMIDE; HAMSTERS; LUMINESCENT PROTEINS/METABOLISM; MICE; MOLECULAR SEQUENCE DATA; OCTOXYNOL; ORGANELLES/METABOLISM; SUBCELLULAR FRACTIONS; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; NASA DISCIPLINE NEUROSCIENCE; NON-NASA CENTER
Availability Source: Other Sources
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