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Effects of 2-deoxy-D-glucose administration on cytokine production in BDF1 micePhysical exercise and diet changes have been shown to affect immune parameters, and similar effects are also induced by the administration of a nonmetabolizable glucose analog, 2-deoxy-D-glucose (2-DG). The present study was designed to characterize the effects of glucoprivation induced by 2-DG administration on concentrations of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-6 in the blood and interferon-gamma (IFN-gamma), IL-2, and IL-4 in vitro production by partially purified T splenocytes in BDF1 mice. Mice (n = 8 per group) were injected intraperitoneally one or three times with 0, 500, 750, or 1000 mg/kg of 2-DG, and blood and spleens were collected 2 h after the last injection. Partially purified T splenocytes were cultured 24 h in the presence of concanavalin A (ConA). A significant increase in the corticosterone levels with the amount of 2-DG injected was observed after one or three injections (p<0.05). The amount of 2-DG injected was associated with an increase in TNF-alpha, IL-1beta, and IL-6 concentrations in the blood of mice after one or three injections of 2-DG (p<0.05). A significant decrease in in vitro proliferation of partially purified splenocytes in the presence of ConA was associated with a decrease in IFN-gamma production in the culture supernatants and an increase in IL-1 receptor expression on the cell surface (p<0.05).
Document ID
20040141636
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Dreau, D. (Carolinas Medical Center Charlotte, NC 28232, United States)
Morton, D. S.
Foster, M.
Fowler, N.
Sonnenfeld, G.
Date Acquired
August 22, 2013
Publication Date
February 1, 2000
Publication Information
Publication: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
Volume: 20
Issue: 2
ISSN: 1079-9907
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Regulatory Physiology
NASA Program Fundamental Space Biology
Non-NASA Center