NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Androgen and taxol cause cell type-specific alterations of centrosome and DNA organization in androgen-responsive LNCaP and androgen-independent DU145 prostate cancer cellsWe investigated the effects of androgen and taxol on the androgen-responsive LNCaP and androgen-independent DU145 prostate cancer cell lines. Cells were treated for 48 and 72 h with 0.05-1 nM of the synthetic androgen R1881 and with 100 nM taxol. Treatment of LNCaP cells with 0.05 nM R1881 led to increased cell proliferation, whereas treatment with 1 nM R1881 resulted in inhibited cell division, DNA cycle arrest, and altered centrosome organization. After treatment with 1 nM R1881, chromatin became clustered, nuclear envelopes convoluted, and mitochondria accumulated around the nucleus. Immunofluorescence microscopy with antibodies to centrosomes showed altered centrosome structure. Although centrosomes were closely associated with the nucleus in untreated cells, they dispersed into the cytoplasm after treatment with 1 nM R1881. Microtubules were only faintly detected in 1 nM R1881-treated LNCaP cells. The effects of taxol included microtubule bundling and altered mitochondria morphology, but not DNA organization. As expected, the androgen-independent prostate cancer cell line DU145 was not affected by R1881. Treatment with taxol resulted in bundling of microtubules in both cell lines. Additional taxol effects were seen in DU145 cells with micronucleation of DNA, an indication of apoptosis. Simultaneous treatment with R1881 and taxol had no additional effects on LNCaP or DU145 cells. These results suggest that LNCaP and DU145 prostate cancer cells show differences not only in androgen responsiveness but in sensitivity to taxol as well. Copyright 2000 Wiley-Liss, Inc.
Document ID
20040141692
Document Type
Reprint (Version printed in journal)
Authors
Schatten, H. (University of Missouri-Columbia Columbia, Missouri 65211, United States)
Ripple, M.
Balczon, R.
Weindruch, R.
Chakrabarti, A.
Taylor, M.
Hueser, C. N.
Date Acquired
August 22, 2013
Publication Date
January 1, 2000
Publication Information
Publication: Journal of cellular biochemistry
Volume: 76
Issue: 3
ISSN: 0730-2312
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: T32AG0002
Distribution Limits
Public
Copyright
Other
Keywords
NASA Program Fundamental Space Biology
Non-NASA Center
NASA Discipline Developmental Biology