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Localization of the human 64kD autoantigen D1 to myofibrils in a subset of extraocular muscle fibersPURPOSE. To evaluate the tissue-specific expression pattern of the 64kD human autoantigen D1, a tropomodulin-related protein that may be involved in thyroid-associated ophthalmopathy. METHODS. Recombinant 64kD human autoantigen D1 was generated in a bacterial expression system and used to immunize rabbits. Specific antibodies were affinity-purified and used for Western blots on normal and hyperthyroid rat and rabbit tissue, and immunofluorescence localization on cryosections of rat tissue. RESULTS. Anti-64kD human autoantigen D1 antibodies recognize specifically a approximately 70kD polypeptide in western blots of extraocular muscle, sternothyroid muscle, and smooth muscle. Immunofluorescence staining demonstrates that the 64kD human autoantigen D1 localizes to myofibrils in slow fibers from rat extraocular and sternothyroid muscle. The level of this protein is not altered in extraocular muscles from hyperthyroid rabbits. CONCLUSIONS. The 64kD human autoantigen D1 is expressed in slow fibers of extraocular and sternothyroid muscles as a component of myofibrils, and is not upregulated in conditions of hyperthyroidism.
Document ID
20040141799
Document Type
Reprint (Version printed in journal)
Authors
Conley, C. A. (The Scripps Research Institute 10550 N. Torrey Pines Rd. CA 92037, La Jolla, United States)
Fowler, V. M.
Date Acquired
August 22, 2013
Publication Date
October 1, 1999
Publication Information
Publication: Current eye research
Volume: 19
Issue: 4
ISSN: 0271-3683
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: GM34225
Distribution Limits
Public
Copyright
Other