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Record Details

Record 20 of 10479
Arabidopsis alcohol dehydrogenase expression in both shoots and roots is conditioned by root growth environment
External Online Source: doi:10.1104/pp.121.2.429
Author and Affiliation:
Chung, H. J.(University of Florida, Program in Plant Molecular and Cellular Biology, Department of Horticultural Sciences, Gainesville, Florida 32611, United States)
Ferl, R. J.
Abstract: It is widely accepted that the Arabidopsis Adh (alcohol dehydrogenase) gene is constitutively expressed at low levels in the roots of young plants grown on agar media, and that the expression level is greatly induced by anoxic or hypoxic stresses. We questioned whether the agar medium itself created an anaerobic environment for the roots upon their growing into the gel. beta-Glucuronidase (GUS) expression driven by the Adh promoter was examined by growing transgenic Arabidopsis plants in different growing systems. Whereas roots grown on horizontal-positioned plates showed high Adh/GUS expression levels, roots from vertical-positioned plates had no Adh/GUS expression. Additional results indicate that growth on vertical plates closely mimics the Adh/GUS expression observed for soil-grown seedlings, and that growth on horizontal plates results in induction of high Adh/GUS expression that is consistent with hypoxic or anoxic conditions within the agar of the root zone. Adh/GUS expression in the shoot apex is also highly induced by root penetration of the agar medium. This induction of Adh/GUS in shoot apex and roots is due, at least in part, to mechanisms involving Ca2+ signal transduction.
Publication Date: Oct 01, 1999
Document ID:
20040141801
(Acquired Nov 09, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Plant physiology (ISSN 0032-0889); Volume 121; 2; 429-36
Publisher Information: United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ALCOHOLS; DEHYDROGENASES; ENZYMOLOGY; GENE EXPRESSION REGULATION; PLANTS (BOTANY); GENETICALLY MODIFIED PLANTS; GENETICS; PLANT ROOTS; STEMS
Other Descriptors: ALCOHOL DEHYDROGENASE/GENETICS; ARABIDOPSIS/ENZYMOLOGY/GENETICS/GROWTH & DEVELOPMENT; GENE EXPRESSION REGULATION, PLANT; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GLUCURONIDASE/GENETICS; PLANT ROOTS; PLANT SHOOTS; PLANTS, GENETICALLY MODIFIED; SUPPORT, U.S. GOV'T, NON-P.H.S; NASA DISCIPLINE PLANT BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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