NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Differential antimutagenicity of WR-1065 added after irradiation in L5178Y cell linesThe purpose of this study was to determine the antimutagenicity of WR-1065 added after irradiation of cells of cell lines differing in their ability to rejoin radiation-induced DNA double-strand breaks (DSBs). The postirradiation antimutagenicity of WR-1065 at the thymidine kinase locus was demonstrated for L5178Y (LY)-S1 cells that are deficient in repair of DNA DSBs. Less postirradiation antimutagenicity of WR-1065 was observed in LY-R16 and LY-SR1 cells, which are relatively efficient in DSB repair. Postirradiation treatment with WR-1065 had only a small stimulatory effect on DSB rejoining. A 3-h incubation of irradiated LY cells with WR-1065 caused slight changes in the distribution of cells in the phases of the cell cycle that differed between LY-S1 and LY-SR1 cells. Both LY-S1 and LY-SR1 cells were protected against the cytotoxic and mutagenic effects of radiation when WR-1065 was present 30 min before and during the irradiation. We conclude that the differential postirradiation effects of WR-1065 in the LY-S1 and LY-SR1 cells are not caused by differences in cellular uptake of the radioprotector or in its radical scavenging activity. Possible mechanisms for the postirradiation antimutagenicity of WR-1065 are discussed.
Document ID
20040142012
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Evans, H. H.
(Case Western Reserve University School of Medicine Cleveland, Ohio 44106-4942, United States)
Horng, M. F.
Ricanati, M.
McCoy, E. C.
Date Acquired
August 22, 2013
Publication Date
April 1, 1999
Publication Information
Publication: Radiation research
Volume: 151
Issue: 4
ISSN: 0033-7587
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Radiation Health
Non-NASA Center

Available Downloads

There are no available downloads for this record.
No Preview Available