NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Identification of a dual-specificity protein phosphatase that inactivates a MAP kinase from ArabidopsisMitogen-activated protein kinases (MAPKs) play a key role in plant responses to stress and pathogens. Activation and inactivation of MAPKs involve phosphorylation and dephosphorylation on both threonine and tyrosine residues in the kinase domain. Here we report the identification of an Arabidopsis gene encoding a dual-specificity protein phosphatase capable of hydrolysing both phosphoserine/threonine and phosphotyrosine in protein substrates. This enzyme, designated AtDsPTP1 (Arabidopsis thaliana dual-specificity protein tyrosine phosphatase), dephosphorylated and inactivated AtMPK4, a MAPK member from the same plant. Replacement of a highly conserved cysteine by serine abolished phosphatase activity of AtDsPTP1, indicating a conserved catalytic mechanism of dual-specificity protein phosphatases from all eukaryotes.
Document ID
20040142057
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Gupta, R.
(University of California at Berkeley 94720 United States)
Huang, Y.
Kieber, J.
Luan, S.
Evans, M. L.
Date Acquired
August 22, 2013
Publication Date
December 1, 1998
Publication Information
Publication: The Plant journal : for cell and molecular biology
Volume: 16
Issue: 5
ISSN: 0960-7412
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Plant Biology

Available Downloads

There are no available downloads for this record.
No Preview Available