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Role of heat shock protein Hsp25 in the response of the orofacial nuclei motor system to physiological stressAlthough expression of the small heat shock protein family member Hsp25 has been previously observed in the central nervous system (CNS), both constitutively and upon induction, its function in the CNS remains far from clear. In the present study we have characterized the spatial pattern of expression of Hsp25 in the normal adult mouse brain as well as the changes in expression patterns induced by subjecting mice to experimental hyperthermia or hypoxia. Immunohistochemical analysis revealed a surprisingly restricted pattern of constitutive expression of Hsp25 in the brain, limited to the facial, trigeminal, ambiguus, hypoglossal and vagal motor nuclei of the brainstem. After hyperthermia or hypoxia treatment, significant increases in the levels of Hsp25 were observed in these same areas and also in fibers of the facial and trigeminal nerve tracts. Immunoblot analysis of protein lysates from brainstem also showed the same pattern of induction of Hsp25. Surprisingly, no other area in the brain showed expression of Hsp25, in either control or stressed animals. The highly restricted expression of Hsp25 implies that this protein may have a specific physiological role in the orofacial motor nuclei, which govern precise coordination between muscles of mastication and the pharynx, larynx, and face. Its rapid induction after stress further suggests that Hsp25 may serve as a specific molecular chaperone in the lower cholinergic motor neurons and along their fibers under conditions of stress or injury. Copyright 1998 Elsevier Science B.V.
Document ID
Document Type
Reprint (Version printed in journal)
Murashov, A. K.
(Columbia University College of Physicians and Surgeons Black Bldg. #1613, 630 West 168 Street, New York, NY 10032, United States)
Talebian, S.
Wolgemuth, D. J.
Date Acquired
August 22, 2013
Publication Date
December 10, 1998
Publication Information
Publication: Brain research. Molecular brain research
Volume: 63
Issue: 1
ISSN: 0169-328X
Subject Category
Life Sciences (General)
Distribution Limits
Non-NASA Center
NASA Discipline Developmental Biology

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