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identification of phosphoproteins coupled to initiation of motility in live epididymal mouse spermA method for collecting live immotile cauda epididymal mouse sperm that initiate motility by dilution into an activation buffer is described. Sperm in collection buffer showed low percent motility (MOT) and population progression (PRG) that increased 10-fold and 9-fold, respectively, during the first 2 min after dilution into activation buffer. Western phosphoserine (pS), phosphothreonine (pT), and phosphotyrosine (pY) analysis revealed a 120 kDa protein that markedly increased in pT content during initiation of motility and may be related to FP130, the motility-coupled axonemal protein of sea urchin sperm. A prominent 82 kDa protein that was pS and pT-phosphorylated in immotile and motile sperm is likely the fibrous sheath component AKAP82 that is phosphorylated during spermatogenesis. Analysis of live human sperm also identified a prominent 120 kDa pT protein. Thus it appears that phosphorylation of FP130 and related 120 kDa proteins in mouse, and perhaps human sperm, represent common targets during motility initiation in sperm. Copyright 1998 Academic Press.
Document ID
20040142195
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Tash, J. S.
(University of Kansas Medical Center Kansas City, Kansas, 66160, United States)
Bracho, G. E.
Date Acquired
August 22, 2013
Publication Date
October 20, 1998
Publication Information
Publication: Biochemical and biophysical research communications
Volume: 251
Issue: 2
ISSN: 0006-291X
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: HD-33994
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology