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The nucleus is the target for radiation-induced chromosomal instabilityWe have previously described chromosomal instability in cells of a human-hamster hybrid cell line after exposure to X rays. Chromosomal instability in these cells is characterized by the appearance of novel chromosomal rearrangements multiple generations after exposure to ionizing radiation. To identify the cellular target(s) for radiation-induced chromosomal instability, cells were treated with 125I-labeled compounds and frozen. Radioactive decays from 125I cause damage to the cell primarily at the site of their decay, and freezing the cells allows damage to accumulate in the absence of other cellular processes. We found that the decay of 125I-iododeoxyuridine, which is incorporated into the DNA, caused chromosomal instability. While cell killing and first-division chromosomal rearrangements increased with increasing numbers of 125I decays, the frequency of chromosomal instability was independent of dose. Chromosomal instability could also be induced from incorporation of 125I-iododeoxyuridine without freezing the cells for accumulation of decays. This indicates that DNA double-strand breaks in frozen cells resulting from 125I decays failed to lead to instability. Incorporation of an 125I-labeled protein (125I-succinyl-concanavalin A), which was internalized into the cell and/or bound to the plasma membrane, neither caused chromosomal instability nor potentiated chromosomal instability induced by 125I-iododeoxyuridine. These results show that the target for radiation-induced chromosomal instability in these cells is the nucleus.
Document ID
20040142215
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Kaplan, M. I.
(University of California San Francisco, 94143-0750, United States)
Morgan, W. F.
Date Acquired
August 22, 2013
Publication Date
October 1, 1998
Publication Information
Publication: Radiation research
Volume: 150
Issue: 4
ISSN: 0033-7587
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA 73924
CONTRACT_GRANT: GM54189
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Radiation Health

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