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Long term organ culture of human prostate tissue in a NASA-designed rotating wall bioreactorPURPOSE: To maintain ex vivo integral prostatic tissue including intact stromal and ductal elements using the NASA-designed Rotating Wall Vessel (RWV) which maintains colocalized cells in an environment that promotes both three-dimensional cellular interactions together with the uniform mass transfer of nutrients and metabolic wastes. MATERIALS AND METHODS: Samples of normal prostate were obtained as a byproduct of transurethral prostatectomy or needle biopsy. Prostatic tissue dissected into small 1 x 1 mm. blocks was cultured in the Rotating Wall Vessel (RWV) Bioreactor for various time periods and analyzed using histological, immunochemical, and total cell RNA assays. RESULTS: We report the long term maintenance of benign explanted human prostate tissue grown in simple culture medium, under the simulated microgravity conditions afforded by the RWV bioreactor. Mesenchymal stromal elements including blood vessels and architecturally preserved tubuloglandular acini were maintained for a minimum of 28 days. Cytokeratins, vimentin and TGF-beta2 receptor and ligand were preserved through the entire culture period as revealed by immunocytochemistry. Prostatic acid phosphatase (PAP) was continuously expressed during the culture period, although somewhat decreased. Prostatic specific antigen (PSA) and its transcript were down regulated over time of culture. Prostatic carcinoma cells from the TSU cell line were able to invade RWV-cultured benign prostate tissue explants. CONCLUSIONS: The RWV bioreactor represents an additional new technology for culturing prostate tissue for further investigations concerning the basic physiology and pathobiology of this clinically important tissue.
Document ID
20040151146
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Margolis, L.
(NASA Headquarters Washington, DC United States)
Hatfill, S.
Chuaqui, R.
Vocke, C.
Emmert-Buck, M.
Linehan, W. M.
Duray, P. H.
Date Acquired
August 22, 2013
Publication Date
January 1, 1999
Publication Information
Publication: The Journal of urology
Volume: 161
Issue: 1
ISSN: 0022-5347
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other

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