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Csa-19, a radiation-responsive human gene, identified by an unbiased two-gel cDNA library screening method in human cancer cellsA novel polymerase chain reaction (PCR)-based method was used to identify candidate genes whose expression is altered in cancer cells by ionizing radiation. Transcriptional induction of randomly selected genes in control versus irradiated human HL60 cells was compared. Among several complementary DNA (cDNA) clones recovered by this approach, one cDNA clone (CL68-5) was downregulated in X-irradiated HL60 cells but unaffected by 12-O-tetradecanoyl phorbol-13-acetate, forskolin, or cyclosporin-A. DNA sequencing of the CL68-5 cDNA revealed 100% nucleotide sequence homology to the reported human Csa-19 gene. Northern blot analysis of RNA from control and irradiated cells revealed the expression of a single 0.7-kilobase (kb) messenger RNA (mRNA) transcript. This 0.7-kb Csa-19 mRNA transcript was also expressed in a variety of human adult and corresponding fetal normal tissues. Moreover, when the effect of X- or fission neutron-irradiation on Csa-19 mRNA was compared in cultured human cells differing in p53 gene status (p53-/- versus p53+/+), downregulation of Csa-19 by X-rays or fission neutrons was similar in p53-wild type and p53-null cell lines. Our results provide the first known example of a radiation-responsive gene in human cancer cells whose expression is not associated with p53, adenylate cyclase or protein kinase C.
Document ID
20040166049
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Balcer-Kubiczek, E. K.
(University of Maryland Baltimore 21201, United States)
Meltzer, S. J.
Han, L. H.
Zhang, X. F.
Shi, Z. M.
Harrison, G. H.
Abraham, J. M.
Date Acquired
August 22, 2013
Publication Date
June 26, 1997
Publication Information
Publication: Oncogene
Volume: 14
Issue: 25
ISSN: 0950-9232
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: DK47717
CONTRACT_GRANT: CA67497
CONTRACT_GRANT: ES07120
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Radiation Health
Non-NASA Center

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