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Record 42 of 556
Csa-19, a radiation-responsive human gene, identified by an unbiased two-gel cDNA library screening method in human cancer cells
External Online Source: doi:10.1038/sj.onc.1201151
Author and Affiliation:
Balcer-Kubiczek, E. K.(University of Maryland, Department of Radiation Oncology, Baltimore 21201, United States)
Meltzer, S. J.
Han, L. H.
Zhang, X. F.
Shi, Z. M.
Harrison, G. H.
Abraham, J. M.
Abstract: A novel polymerase chain reaction (PCR)-based method was used to identify candidate genes whose expression is altered in cancer cells by ionizing radiation. Transcriptional induction of randomly selected genes in control versus irradiated human HL60 cells was compared. Among several complementary DNA (cDNA) clones recovered by this approach, one cDNA clone (CL68-5) was downregulated in X-irradiated HL60 cells but unaffected by 12-O-tetradecanoyl phorbol-13-acetate, forskolin, or cyclosporin-A. DNA sequencing of the CL68-5 cDNA revealed 100% nucleotide sequence homology to the reported human Csa-19 gene. Northern blot analysis of RNA from control and irradiated cells revealed the expression of a single 0.7-kilobase (kb) messenger RNA (mRNA) transcript. This 0.7-kb Csa-19 mRNA transcript was also expressed in a variety of human adult and corresponding fetal normal tissues. Moreover, when the effect of X- or fission neutron-irradiation on Csa-19 mRNA was compared in cultured human cells differing in p53 gene status (p53-/- versus p53+/+), downregulation of Csa-19 by X-rays or fission neutrons was similar in p53-wild type and p53-null cell lines. Our results provide the first known example of a radiation-responsive gene in human cancer cells whose expression is not associated with p53, adenylate cyclase or protein kinase C.
Publication Date: Jun 26, 1997
Document ID:
20040166049
(Acquired Nov 23, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Oncogene; p. 3051-7; (ISSN 0950-9232); Volume 14; 25
Publisher Information: United Kingdom
Contract/Grant/Task Num: CA67497; DK47717; ES07120
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: BREAST; CANCER; COMPLEMENTARY DNA; GELS; GENETICS; LIBRARIES; NEOPLASMS; ONCOGENES; AGE FACTOR; CLONING (BIOLOGY); CULTURED CELLS; GENE EXPRESSION REGULATION; GENES; NEUTRONS; RADIATION DOSAGE; RADIATION EFFECTS; TISSUES (BIOLOGY); TUMOR SUPPRESSOR GENES; TUMORS; X RAYS
Other Descriptors: BREAST NEOPLASMS/DRUG THERAPY/GENETICS/RADIOTHERAPY; HL-60 CELLS/DRUG EFFECTS/PHYSIOLOGY/RADIATION EFFECTS; ONCOGENES/DRUG EFFECTS/GENETICS/RADIATION EFFECTS; AGE FACTORS; BLOTTING, NORTHERN; CLONING, MOLECULAR; COMPARATIVE STUDY; CYCLOSPORINE/PHARMACOLOGY; DNA, COMPLEMENTARY; DOSE-RESPONSE RELATIONSHIP, RADIATION; DOWN-REGULATION; FORSKOLIN/PHARMACOLOGY; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENE LIBRARY; GENES, P53/DRUG EFFECTS/RADIATION EFFECTS; HUMAN; MOLECULAR SEQUENCE DATA; NEUTRONS; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; TETRADECANOYLPHORBOL ACETATE/PHARMACOLOGY; TISSUE DISTRIBUTION; TUMOR CELLS, CULTURED; X-RAYS; NASA DISCIPLINE RADIATION HEALTH; NON-NASA CENTER
Availability Source: Other Sources
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