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Chloral hydrate alters the organization of the ciliary basal apparatus and cell organelles in sea urchin embryosThe mitotic inhibitor, chloral hydrate, induces ciliary loss in the early embryo phase of Lytechinus pictus. It causes a breakdown of cilia at the junction of the cilium and the basal body known as the basal plate. This leaves the plasma membrane temporarily unsealed. The basal apparatus accessory structures, consisting of the basal body, basal foot, basal foot cap, striated side arm, and striated rootlet, are either misaligned or disintegrated by treatment with chloral hydrate. Furthermore, microtubules which are associated with the basal apparatus are disassembled. Mitochondria accumulate at the base of cilia - underneath the plasma membrane - and show alterations in their structural organization. The accumulation of mitochondria is observed in 40% of all electron micrograph sections while 60% show the areas mostly devoid of mitochondria. The microvilli surrounding a cilium and striated rootlet remain intact in the presence of chloral hydrate. These results suggest that deciliation in early sea urchin embryos by chloral hydrate is caused by combined effects on the ciliary membrane and on microtubules in the cilia. Furthermore, it is suggested that chloral hydrate can serve as a tool to explore the cytoskeletal mechanisms that are involved in cilia motility in the developing sea urchin embryo.
Document ID
20040172587
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Chakrabarti, A. (University of Missouri-Columbia 1600 East Rollins Street, Columbia, MO 65211, United States)
Schatten, H.
Mitchell, K. D.
Crosser, M.
Taylor, M.
Date Acquired
August 22, 2013
Publication Date
September 1, 1998
Publication Information
Publication: Cell and tissue research
Volume: 293
Issue: 3
ISSN: 0302-766X
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Developmental Biology