NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Identification of sucrose synthase as an actin-binding proteinSeveral lines of evidence indicate that sucrose synthase (SuSy) binds both G- and F-actin: (i) presence of SuSy in the Triton X-100-insoluble fraction of microsomal membranes (i.e. crude cytoskeleton fraction); (ii) co-immunoprecipitation of actin with anti-SuSy monoclonal antibodies; (iii) association of SuSy with in situ phalloidin-stabilized F-actin filaments; and (iv) direct binding to F-actin, polymerized in vitro. Aldolase, well known to interact with F-actin, interfered with binding of SuSy, suggesting that a common or overlapping binding site may be involved. We postulate that some of the soluble SuSy in the cytosol may be associated with the actin cytoskeleton in vivo.
Document ID
20040172609
Document Type
Reprint (Version printed in journal)
Authors
Winter, H. (US Department of Agriculture, Agricultural Research Service Raleigh, NC, United States)
Huber, J. L.
Huber, S. C.
Davies, E.
Date Acquired
August 22, 2013
Publication Date
July 3, 1998
Publication Information
Publication: FEBS letters
Volume: 430
Issue: 3
ISSN: 0014-5793
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Plant Biology