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Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinin in ArabidopsisCytokinins are central regulators of plant growth and development, but little is known about their mode of action. By using differential display, we identified a gene, IBC6 (for induced by cytokinin), from etiolated Arabidopsis seedlings, that is induced rapidly by cytokinin. The steady state level of IBC6 mRNA was elevated within 10 min by the exogenous application of cytokinin, and this induction did not require de novo protein synthesis. IBC6 was not induced by other plant hormones or by light. A second Arabidopsis gene with a sequence highly similar to IBC6 was identified. This IBC7 gene also was induced by cytokinin, although with somewhat slower kinetics and to a lesser extent. The pattern of expression of the two genes was similar, with higher expression in leaves, rachises, and flowers and lower transcript levels in roots and siliques. Sequence analysis revealed that IBC6 and IBC7 are similar to the receiver domain of bacterial two-component response regulators. This homology, coupled with previously published work on the CKI1 histidine kinase homolog, suggests that these proteins may play a role in early cytokinin signaling.
Document ID
20040172651
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Brandstatter, I. (University of Illinois at Chicago Chicago, Illinois 60607, United States)
Kieber, J. J.
Evans, M. L.
Date Acquired
August 22, 2013
Publication Date
June 1, 1998
Publication Information
Publication: The Plant cell
Volume: 10
Issue: 6
ISSN: 1040-4651
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Plant Biology