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Myogenin, MyoD, and myosin expression after pharmacologically and surgically induced hypertrophyThe relationship between myogenin or MyoD expression and hypertrophy of the rat soleus produced either by clenbuterol and 3,3', 5-triiodo-L-thyronine (CT) treatment or by surgical overload was examined. Mature female rats were subjected to surgical overload of the right soleus with the left soleus serving as a control. Another group received the same surgical treatment but were administered CT. Soleus muscles were harvested 4 wk after surgical overload and weighed. Myosin heavy chain isoforms were separated by using polyacrylamide gel electrophoresis while myogenin and MyoD expression were evaluated by Northern analysis. CT and functional overload increased soleus muscle weight. CT treatment induced the appearance of the fast type IIX myosin heavy chain isoform, depressed myogenin expression, and induced MyoD expression. However, functional overload did not alter myogenin or MyoD expression in CT-treated or non-CT-treated rats. Thus pharmacologically and surgically induced hypertrophy have differing effects on myogenin and MyoD expression, because their levels were associated with changes in myosin heavy chain composition (especially type IIX) rather than changes in muscle mass.
Document ID
20040172712
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Mozdziak, P. E.
(University of Wisconsin Medical School Madison, Wisconsin 53706, United States)
Greaser, M. L.
Schultz, E.
Date Acquired
August 22, 2013
Publication Date
April 1, 1998
Publication Information
Publication: Journal of applied physiology (Bethesda, Md. : 1985)
Volume: 84
Issue: 4
ISSN: 8750-7587
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Musculoskeletal
Non-NASA Center

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