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Record 16 of 1340
Changes in soluble sugar, starch, and alcohol dehydrogenase in Arabidopsis thaliana exposed to N2 diluted atmospheres
Author and Affiliation:
Porterfield, D. M.(Louisiana State University Agricultural Center, Department of Plant Pathology and Crop Physiology, Baton Rouge 70803, United States)
Crispi, M. L.
Musgrave, M. E.
Abstract: Proper exchange of atmospheric gases is important for normal root and shoot metabolism in plants. This study was conducted to determine how restricted air supply affects foliar carbohydrates, while using the marker enzyme alcohol dehydrogenase (ADH) to report on the oxygenation status of the rootzone. Fourteen-day-old Arabidopsis thaliana (L.) Heynh. plants grown singly in 7-ml tubes containing agarified nutrient medium were placed in coupled Magenta vessels and exposed for six days to either ambient air or one of six different air/nitrogen dilutions. Redox potential of the agar medium was measured immediately after harvesting and freezing leaf tissue, and then root systems were quickly extracted from the agar and frozen for subsequent analyses. Redox potential measurements indicated that this series of gas mixtures produced a transition from hypoxia to anoxia in the root zones. Root ADH activity increased at higher rates as the redox potential neared anoxic levels. In contrast, ADH mRNA expression quickly neared its maximum as the medium became hypoxic and showed little further increase as it became anoxic. Foliar carbohydrate levels increased 1.5- to 2-fold with decreased availability of metabolic gases, with starch increasing at higher concentrations of air than soluble carbohydrate. The results serve as a model for plant performance under microgravity conditions, where absence of convective air movement prevents replenishment of metabolic gases.
Publication Date: Dec 01, 1997
Document ID:
20040172749
(Acquired Dec 09, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Plant & cell physiology (ISSN 0032-0781); Volume 38; 12; 1354-8
Publisher Information: Japan
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ALCOHOLS; DILUTION; NITROGEN; PLANTS (BOTANY); STARCHES; SUGARS; ATMOSPHERES; CARBOHYDRATES; DEHYDROGENASES; OXIDATION-REDUCTION REACTIONS; PLANT ROOTS; STEMS
Other Descriptors: ARABIDOPSIS/GROWTH & DEVELOPMENT/METABOLISM; NITROGEN/METABOLISM; AGAR; ALCOHOL DEHYDROGENASE/METABOLISM; ATMOSPHERE; CARBOHYDRATES/METABOLISM; OXIDATION-REDUCTION; PLANT ROOTS/METABOLISM; PLANT SHOOTS/METABOLISM; STARCH/METABOLISM; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; NASA DISCIPLINE PLANT BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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