NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Rejoining and misrejoining of radiation-induced chromatin breaks. III. Hypertonic treatmentIt has been shown that treatment in anisotonic medium modifies rejoining of radiation-induced breaks in interphase chromosomes. In previous work, we have demonstrated that formation of exchanges in human lymphocytes has a slow component (half-time of 1-2 h), but a fraction of exchanges are also observed in samples assayed soon after exposure. In this paper we studied the effect of hypertonic treatment on rejoining and misrejoining of radiation-induced breaks using fluorescence in situ hybridization of prematurely condensed chromosomes in human lymphocytes. Isolated lymphocytes were irradiated with 7 Gy gamma rays, fused to mitotic hamster cells and incubated in hypertonic solution (0.5 M NaCl) for the period normally allowed for interphase chromosome condensation to occur. The data from hypertonic treatment experiments indicate the presence of a class of interphase chromosome breaks that rejoin and misrejoin very quickly (half-time of 5-6 min). The fast misrejoining of these lesions is considered to be responsible for the initial level of exchanges which we reported previously. No significant effect of hypertonic treatment on the yield of chromosome aberrations scored at the first postirradiation mitosis was detected.
Document ID
20040172818
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Durante, M.
(Universita Federico II Napoli, Italy)
George, K.
Wu, H. L.
Yang, T. C.
Date Acquired
August 22, 2013
Publication Date
January 1, 1998
Publication Information
Publication: Radiation research
Volume: 149
Issue: 1
ISSN: 0033-7587
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Radiation Health
NASA Center JSC

Available Downloads

There are no available downloads for this record.
No Preview Available