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Biodosimetry of ionizing radiation by selective painting of prematurely condensed chromosomes in human lymphocytesPainting of interphase chromosomes can be useful for biodosimetric purposes in particular cases such as radiation therapy, accidental exposure to very high radiation doses and exposure to densely ionizing radiation, for example during space missions. Biodosimetry of charged-particle radiation is analyzed in the present paper. Target cells were human peripheral blood lymphocytes irradiated in vitro with gamma rays, protons and iron ions. After exposure, lymphocytes were incubated for different times to allow repair of radiation-induced damage and then fused to mitotic hamster cells to promote premature condensation in the interphase chromosomes. Chromosome spreads were then hybridized with whole-chromosome DNA probes labeled with fluorescent stains. Dose-response curves for the induction of chromatin fragments shortly after exposure, as well as the kinetics of rejoining and misrejoining, were not markedly dependent on linear energy transfer. However, after exposure to heavy ions, more aberrations were scored in the interphase cells after incubation for repair than in metaphase samples harvested at the first postirradiation mitosis. On the other hand, no significant differences were observed in the two samples after exposure to sparsely ionizing radiation. These results suggest that interphase chromosome painting can be a useful tool for biodosimetry of particle radiation.
Document ID
20040172868
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Durante, M.
(NASA Johnson Space Center Houston, TX United States)
George, K.
Yang, T. C.
Date Acquired
August 22, 2013
Publication Date
November 1, 1997
Publication Information
Publication: Radiation research
Volume: 148
Issue: 5 Suppl
ISSN: 0033-7587
Subject Category
Life Sciences (General)
Distribution Limits
Public
Copyright
Other
Keywords
NASA Center JSC
NASA Discipline Radiation Health

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