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Record 7 of 311
Neonatal rat heart cells cultured in simulated microgravity
Author and Affiliation:
Akins, R. E.(Alfred I. duPont Institute, Department of Medical Cell Biology, Nemours Research Programs, Wilmington, Delaware 19899, United States)
Schroedl, N. A.
Gonda, S. R.
Hartzell, C. R.
Abstract: In vitro characteristics of cardiac cells cultured in simulated microgravity are reported. Tissue culture methods performed at unit gravity constrain cells to propagate, differentiate, and interact in a two-dimensional (2D) plane. Neonatal rat cardiac cells in 2D culture organize predominantly as bundles of cardiomyocytes with the intervening areas filled by nonmyocyte cell types. Such cardiac cell cultures respond predictably to the addition of exogenous compounds, and in many ways they represent an excellent in vitro model system. The gravity-induced 2D organization of the cells, however, does not accurately reflect the distribution of cells in the intact tissue. We have begun characterizations of a three-dimensional (3D) culturing system designed to mimic microgravity. The NASA-designed High-Aspect Ratio Vessel (HARV) bioreactors provide a low shear environment that allows cells to be cultured in static suspension. HARV-3D cultures were prepared on microcarrier beads and compared to control-2D cultures using a combination of microscopic and biochemical techniques. Both systems were uniformly inoculated and medium exchanged at standard intervals. Cells in control cultures adhered to the polystyrene surface of the tissue culture dishes and exhibited typical 2D organization. Cells cultured in HARVs adhered to microcarrier beads, the beads aggregated into defined clusters containing 8 to 15 beads per cluster, and the clusters exhibited distinct 3D layers: myocytes and fibroblasts appeared attached to the surfaces of beads and were overlaid by an outer cell type. In addition, cultures prepared in HARVs using alternative support matrices also displayed morphological formations not seen in control cultures. Generally, the cells prepared in HARV and control cultures were similar; however, the dramatic alterations in 3D organization recommend the HARV as an ideal vessel for the generation of tissuelike organization of cardiac cells in vitro.
Publication Date: May 01, 1997
Document ID:
20040172968
(Acquired Dec 09, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: In vitro cellular & developmental biology. Animal (ISSN 1071-2690); Volume 33; 5; 337-43
Publisher Information: United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: CULTURED CELLS; CYTOLOGY; HEART; MICROGRAVITY; MYOCARDIUM; RATS; WEIGHTLESSNESS; ANIMALS; CARBON DIOXIDE; CELL DIVISION; OXYGEN; SPACE ENVIRONMENT SIMULATION
Other Descriptors: CELLS, CULTURED; MYOCARDIUM/CYTOLOGY/METABOLISM; WEIGHTLESSNESS; ANIMALS; ANIMALS, NEWBORN; CARBON DIOXIDE; CELL DIVISION; OXYGEN; RATS; SPACE SIMULATION; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; NASA DISCIPLINE MUSCULOSKELETAL; NON-NASA CENTER
Availability Source: Other Sources
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