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Record 3 of 433
Spaceflight exposure effects on transcription, activity, and localization of alcohol dehydrogenase in the roots of Arabidopsis thaliana
External Online Source: doi:10.1104/pp.113.3.685
Author and Affiliation:
Porterfield, D. M.(Louisiana State University Agricultural Center, Department of Plant Pathology and Crop Physiology, Baton Rouge 70803, United States)
Matthews, S. W.
Daugherty, C. J.
Musgrave, M. E.
Abstract: Although considerable research and speculation have been directed toward understanding a plant's perception of gravity and the resulting gravitropic responses, little is known about the role of gravity-dependent physical processes in normal physiological function. These studies were conducted to determine whether the roots of plants exposed to spaceflight conditions may be experiencing hypoxia. Arabidopsis thaliana (L.) Heynh. plants were grown in agar medium during 6 or 11 d of spaceflight exposure on shuttle missions STS-54 (CHROMEX-03) and STS-68 (CHROMEX-05), respectively. The analysis included measurement of agar redox potential and root alcohol dehydrogenase (ADH) activity, localization, and expression. ADH activity increased by 89% as a result of spaceflight exposure for both CHROMEX-03 and -05 experiments, and ADH RNase protection assays revealed a 136% increase in ADH mRNA. The increase in ADH activity associated with the spaceflight roots was realized by a 28% decrease in oxygen availability in a ground-based study; however, no reduction in redox potential was observed in measurements of the spaceflight bulk agar. Spaceflight exposure appears to effect a hypoxic response in the roots of agar-grown plants that may be caused by changes in gravity-mediated fluid and/or gas behavior.
Publication Date: Mar 01, 1997
Document ID:
20040173015
(Acquired Dec 09, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Plant physiology; p. 685-93; (ISSN 0032-0889); Volume 113; 3
Publisher Information: United States
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: ALCOHOLS; DEHYDROGENASES; ENZYMOLOGY; EXPOSURE; PLANT ROOTS; PLANTS (BOTANY); POSITION (LOCATION); TRANSCRIPTION (GENETICS); GENETICS; SPACE SHUTTLE MISSIONS
Other Descriptors: ALCOHOL DEHYDROGENASE/GENETICS/METABOLISM; ARABIDOPSIS/ENZYMOLOGY/PHYSIOLOGY; PLANT ROOTS/ENZYMOLOGY; SPACE FLIGHT; TRANSCRIPTION, GENETIC; SUPPORT, U.S. GOV'T, NON-P.H.S; NASA DISCIPLINE PLANT BIOLOGY; NON-NASA CENTER; FLIGHT EXPERIMENT; STS-54 SHUTTLE PROJECT; STS-68 SHUTTLE PROJECT; MANNED; SHORT DURATION
Availability Source: Other Sources
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