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Activation of the adenylyl cyclase/cyclic AMP/protein kinase A pathway in endothelial cells exposed to cyclic strainThe aim of this study was to assess the involvement of the adenylyl cyclase/cyclic AMP/protein kinase A pathway (AC) in endothelial cells (EC) exposed to different levels of mechanical strain. Bovine aortic EC were seeded to confluence on flexible membrane-bottom wells. The membranes were deformed with either 150 mm Hg (average 10% strain) or 37.5 mm Hg (average 6% strain) vacuum at 60 cycles per minute (0.5 s strain; 0.5 s relaxation) for 0-60 min. The results demonstrate that at 10% average strain (but not 6% average strain) there was a 1.5- to 2.2-fold increase in AC, cAMP, and PKA activity by 15 min when compared to unstretched controls. Further studies revealed an increase in cAMP response element binding protein in EC subjected to the 10% average strain (but not 6% average strain). These data support the hypothesis that cyclic strain activates the AC/cAMP/PKA signal transduction pathway in EC which may occur by exceeding a strain threshold and suggest that cyclic strain may stimulate the expression of genes containing cAMP-responsive promoter elements.
Document ID
20040173023
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Cohen, C. R. (Yale University School of Medicine 333 Cedar Street, New Haven, Connecticut, 06510, United States)
Mills, I.
Du, W.
Kamal, K.
Sumpio, B. E.
Date Acquired
August 22, 2013
Publication Date
February 25, 1997
Publication Information
Publication: Experimental cell research
Volume: 231
Issue: 1
ISSN: 0014-4827
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: RO1 47345
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cell Biology