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Epidemiology of Staphylococcus aureus during space flightStaphylococcus aureus was isolated over 2 years from Space Shuttle mission crewmembers to determine dissemination and retention of bacteria. Samples before and after each mission were from nasal, throat, urine, and feces and from air and surface sampling of the Space Shuttle. DNA fingerprinting of samples by digestion of DNA with SmaI restriction endonuclease followed by pulsed-field gel electrophoresis showed S. aureus from each crewmember had a unique fingerprint and usually only one strain was carried by an individual. There was only one instance of transfer between crewmembers. Strains from interior surfaces after flight matched those of crewmembers, suggesting microbial fingerprinting may have forensic application.
Document ID
20040173080
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Pierson, D. L.
(NASA Johnson Space Center Houston, TX United States)
Chidambaram, M.
Heath, J. D.
Mallary, L.
Mishra, S. K.
Sharma, B.
Weinstock, G. M.
Date Acquired
August 22, 2013
Publication Date
December 31, 1996
Publication Information
Publication: FEMS immunology and medical microbiology
Volume: 16
Issue: 3-4
ISSN: 0928-8244
Subject Category
Man/System Technology And Life Support
Distribution Limits
Public
Copyright
Other
Keywords
Flight Experiment
Non-NASA Center
NASA Center JSC
STS Shuttle Project
short duration
NASA Discipline Environmental Health
NASA Discipline Number 04-10
NASA Program Environmental Health
manned

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