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Cell proliferation and hair cell addition in the ear of the goldfish, Carassius auratusCell proliferation and hair cell addition have not been studied in the ears of otophysan fish, a group of species who have specialized hearing capabilities. In this study we used the mitotic S-phase marker bromodeoxyuridine (BrdU) to identify proliferating cells in the ear of one otophysan species, Carassius auratus (the goldfish). Animals were sacrificed at 3 h or 5 days postinjection with BrdU and processed for immunocytochemistry. The results of the study show that cell proliferation occurs in all of the otic endorgans and results in the addition of new hair cells. BrdU-labeled cells were distributed throughout all epithelia, including the primary auditory endorgan (saccule), where hair cell phenotypes vary considerably along the rostrocaudal axis. This study lays the groundwork for our transmission electron microscopy study of proliferative cells in the goldfish ear (Presson et al., Hearing Research 100 (1996) 10-20) as well as future studies of hair cell development in this species. The ability to predict, based on epithelial location, the future phenotype of developing hair cells in the saccule of the goldfish make that endorgan a particularly powerful model system for the investigation of early hair cell differentiation.
Document ID
20040173122
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Lanford, P. J.
(College Park 20742-4415 United States)
Presson, J. C.
Popper, A. N.
Date Acquired
August 22, 2013
Publication Date
October 1, 1996
Publication Information
Publication: Hearing research
Volume: 100
Issue: 2-Jan
ISSN: 0378-5955
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: 5 T32 DC-00046-02
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Number 40-10
Non-NASA Center
NASA Discipline Neuroscience
NASA Program Space Biology

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