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Record Details

Record 34 of 683
Yeast respond to hypotonic shock with a calcium pulse
External Online Source: doi:10.1074/jbc.271.38.23357
Author and Affiliation:
Batiza, A. F.(University of Wisconsin-Madison, Laboratory of Genetics, Madison, Wisconsin 53706, United States)
Schulz, T.
Masson, P. H.
Abstract: We have used the transgenic AEQUORIN calcium reporter system to monitor the cytosolic calcium ([Ca2+]cyt) response of Saccharomyces cerevisiae to hypotonic shock. Such a shock generates an almost immediate and transient rise in [Ca2+]cyt which is eliminated by gadolinium, a blocker of stretch-activated channels. In addition, this transient rise in [Ca2+]cyt is initially insensitive to 1,2-bis-(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), an extracellular calcium chelator. However, BAPTA abruptly attenuates the maintenance of that transient rise. These data show that hypotonic shock generates a stretch-activated channel-dependent calcium pulse in yeast. They also suggest that the immediate calcium influx is primarily generated from intracellular stores, and that a sustained increase in [Ca2+]cyt depends upon extracellular calcium.
Publication Date: Sep 20, 1996
Document ID:
20040173144
(Acquired Dec 09, 2004)
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: The Journal of biological chemistry (ISSN 0021-9258); Volume 271; 38; 23357-62
Publisher Information: United States
Contract/Grant/Task Num: GM48053
Description: In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: CALCIUM; SACCHAROMYCES; YEAST; GADOLINIUM; GENES; GENETIC ENGINEERING; GENETICS; ION CHANNELS (BIOLOGY); OSMOSIS; PLANTS (BOTANY); PROTEINS; SIGNAL TRANSMISSION; TIME DEPENDENCE
Other Descriptors: CALCIUM/METABOLISM; CYTOSOL/METABOLISM; SACCHAROMYCES CEREVISIAE/METABOLISM; SECOND MESSENGER SYSTEMS; AEQUORIN/GENETICS/METABOLISM; CALCIUM CHANNEL BLOCKERS; GADOLINIUM/PHARMACOLOGY; GENES, REPORTER; OSMOTIC PRESSURE; RECOMBINANT PROTEINS/METABOLISM; SIGNAL TRANSDUCTION; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S; SUPPORT, U.S. GOV'T, P.H.S; TIME FACTORS; NASA DISCIPLINE PLANT BIOLOGY; NON-NASA CENTER
Availability Source: Other Sources
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