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Cellular and molecular analysis of mutagenesis induced by charged particles of defined linear energy transferMutation induction by charged particles of defined linear energy transfer (LET) and gamma rays was scored using human-hamster hybrid AL cells. The LET values for charged particles accelerated at the Radiological Research Accelerator Facility ranged from 10 keV/microm protons to 150 keV/microm 4He ions. The induced mutant fractions at both the S1 and HGPRT loci were dependent on the dose and LET. In addition, for each dose examined, the mutant yield at the S1 locus was 30-60 fold higher than at the corresponding HGPRT locus. To determine whether the mutation spectrum was comparably dependent on dose and LET, independent S1- and HGPRT- mutants induced by 150 keV/microm 4He ions and gamma rays were isolated, and their DNA was analyzed by both Southern blotting and multiplex PCR methods. While the majority of radiation-induced mutants showed deletions of varying sizes, the relative percentage of large deletions was found to be related to both the dose and LET of the radiation examined. Using a mutation system that can detect multilocus changes, results of the present study show that radiation-induced chromosomal loss can be in the millions of base pairs.
Document ID
20040173257
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Zhu, L. X.
(College of Physicians and Surgeons, Columbia University New York 10032, United States)
Waldren, C. A.
Vannias, D.
Hei, T. K.
Chatterjee, A.
Date Acquired
August 22, 2013
Publication Date
March 1, 1996
Publication Information
Publication: Radiation research
Volume: 145
Issue: 3
ISSN: 0033-7587
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: CA49062
CONTRACT_GRANT: CA12536
CONTRACT_GRANT: CA36447
Distribution Limits
Public
Copyright
Other
Keywords
NASA Discipline Radiation Health
Non-NASA Center

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