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Cloning and characterization of the murine homolog of the sno proto-oncogene reveals a novel splice variantThe cellular function(s) of the SNO protein remain undefined. To gain a better understanding of possible developmental roles of this cellular proto-oncogene, we have cloned two murine sno cDNAs and have investigated their expression patterns in embryonic and postnatal tissues. A single major transcript of 7.5 kb is detected in multiple tissues by Northern blot. However, reverse transcriptase polymerase chain reaction (RT-PCR) and RNAse protection assays revealed a novel splice variant in every tissue examined. Two isoforms, termed sno N and sno-dE3 (dE3, deletion within exon 3), were identified. The sno-dE3 isoform employs a novel 5' splice site located within the coding region of the third exon and deletes potential kinase recognition motifs. Transcripts of both sno isoforms accumulate ubiquitously but are most abundant in the developing central nervous system. The in situ hybridization patterns of sno expression during murine development suggest potential roles in tissues with a high degree of cellular proliferation. Expression in terminally differentiated tissues such as muscle and neurons indicates that SNO may have multiple functional activities.
Document ID
20040173273
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Pelzer, T.
(Molecular Cardiology Laboratories, University of Texas Southwestern Medical Center Dallas 75235, United States)
Lyons, G. E.
Kim, S.
Moreadith, R. W.
Blomqvist, C. G.
Date Acquired
August 22, 2013
Publication Date
February 1, 1996
Publication Information
Publication: Developmental dynamics : an official publication of the American Association of Anatomists
Volume: 205
Issue: 2
ISSN: 1058-8388
Subject Category
Life Sciences (General)
Funding Number(s)
CONTRACT_GRANT: HD29471
Distribution Limits
Public
Copyright
Other
Keywords
Non-NASA Center
NASA Discipline Cardiopulmonary

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